Indium Tin Oxide devices for amperometric detection of vesicular release by single cells.
Identifieur interne : 000C80 ( Main/Exploration ); précédent : 000C79; suivant : 000C81Indium Tin Oxide devices for amperometric detection of vesicular release by single cells.
Auteurs : RBID : pubmed:22257976English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Collagen, Tin Compounds.
- instrumentation : Electrochemical Techniques, Single-Cell Analysis.
- Animals, Cell Line, Equipment Design, Exocytosis, Humans, Microelectrodes, Microtechnology, Signal-To-Noise Ratio, Surface Properties.
Abstract
The microfabrication and successful testing of a series of three ITO (Indium Tin Oxide) microsystems for amperometric detection of cells exocytosis are reported. These microdevices have been optimized in order to simultaneously (i) enhance signal-to-noise ratios, as required electrochemical monitoring, by defining appropriate electrodes geometry and size, and (ii) provide surface conditions which allow cells to be cultured over during one or two days, through apposite deposition of a collagen film. The intrinsic electrochemical quality of the microdevices as well as the effect of different collagen treatments were assessed by investigating the voltammetric responses of two classical redox systems, Ru(NH(3))(6)(3+/2+) and Fe(CN)(6)(3-/4-). This established that a moderate collagen treatment does not incur any significant alteration of voltammetric responses or degradation of the excellent signal-to-noise ratio. Among these three microdevices, the most versatile one involved a configuration in which the ITO microelectrodes were delimited by a microchannel coiled into a spiral. Though providing extremely good electrochemical responses this specific design allowed proper seeding and culture of cells permitting either single cell or cell cluster stimulation and analysis.
DOI: 10.1016/j.bpc.2011.12.002
PubMed: 22257976
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Indium Tin Oxide devices for amperometric detection of vesicular release by single cells.</title><author><name sortKey="Meunier, Anne" uniqKey="Meunier A">Anne Meunier</name><affiliation wicri:level="1"><nlm:affiliation>Ecole Normale Supérieure, Département de Chimie, UMR CNRS-ENS-UPMC « PASTEUR », Paris, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Ecole Normale Supérieure, Département de Chimie, UMR CNRS-ENS-UPMC « PASTEUR », Paris</wicri:regionArea><placeName><region type="région">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Fulcrand, Remy" uniqKey="Fulcrand R">Rémy Fulcrand</name></author><author><name sortKey="Darchen, Francois" uniqKey="Darchen F">François Darchen</name></author><author><name sortKey="Guille Collignon, Manon" uniqKey="Guille Collignon M">Manon Guille Collignon</name></author><author><name sortKey="Lemaitre, Frederic" uniqKey="Lemaitre F">Frédéric Lemaître</name></author><author><name sortKey="Amatore, Christian" uniqKey="Amatore C">Christian Amatore</name></author></titleStmt><publicationStmt><date when="2012">2012</date><idno type="doi">10.1016/j.bpc.2011.12.002</idno><idno type="RBID">pubmed:22257976</idno><idno type="pmid">22257976</idno><idno type="wicri:Area/Main/Corpus">000F53</idno><idno type="wicri:Area/Main/Curation">000F53</idno><idno type="wicri:Area/Main/Exploration">000C80</idno></publicationStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Collagen (chemistry)</term><term>Electrochemical Techniques (instrumentation)</term><term>Equipment Design</term><term>Exocytosis</term><term>Humans</term><term>Microelectrodes</term><term>Microtechnology</term><term>Signal-To-Noise Ratio</term><term>Single-Cell Analysis (instrumentation)</term><term>Surface Properties</term><term>Tin Compounds (chemistry)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Collagen</term><term>Tin Compounds</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Electrochemical Techniques</term><term>Single-Cell Analysis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Equipment Design</term><term>Exocytosis</term><term>Humans</term><term>Microelectrodes</term><term>Microtechnology</term><term>Signal-To-Noise Ratio</term><term>Surface Properties</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The microfabrication and successful testing of a series of three ITO (Indium Tin Oxide) microsystems for amperometric detection of cells exocytosis are reported. These microdevices have been optimized in order to simultaneously (i) enhance signal-to-noise ratios, as required electrochemical monitoring, by defining appropriate electrodes geometry and size, and (ii) provide surface conditions which allow cells to be cultured over during one or two days, through apposite deposition of a collagen film. The intrinsic electrochemical quality of the microdevices as well as the effect of different collagen treatments were assessed by investigating the voltammetric responses of two classical redox systems, Ru(NH(3))(6)(3+/2+) and Fe(CN)(6)(3-/4-). This established that a moderate collagen treatment does not incur any significant alteration of voltammetric responses or degradation of the excellent signal-to-noise ratio. Among these three microdevices, the most versatile one involved a configuration in which the ITO microelectrodes were delimited by a microchannel coiled into a spiral. Though providing extremely good electrochemical responses this specific design allowed proper seeding and culture of cells permitting either single cell or cell cluster stimulation and analysis.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">22257976</PMID><DateCreated><Year>2012</Year><Month>02</Month><Day>20</Day></DateCreated><DateCompleted><Year>2012</Year><Month>06</Month><Day>12</Day></DateCompleted><DateRevised><Year>2013</Year><Month>02</Month><Day>22</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-4200</ISSN><JournalIssue CitedMedium="Internet"><Volume>162</Volume><PubDate><Year>2012</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Biophysical chemistry</Title><ISOAbbreviation>Biophys. Chem.</ISOAbbreviation></Journal><ArticleTitle>Indium Tin Oxide devices for amperometric detection of vesicular release by single cells.</ArticleTitle><Pagination><MedlinePgn>14-21</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.bpc.2011.12.002</ELocationID><Abstract><AbstractText>The microfabrication and successful testing of a series of three ITO (Indium Tin Oxide) microsystems for amperometric detection of cells exocytosis are reported. These microdevices have been optimized in order to simultaneously (i) enhance signal-to-noise ratios, as required electrochemical monitoring, by defining appropriate electrodes geometry and size, and (ii) provide surface conditions which allow cells to be cultured over during one or two days, through apposite deposition of a collagen film. The intrinsic electrochemical quality of the microdevices as well as the effect of different collagen treatments were assessed by investigating the voltammetric responses of two classical redox systems, Ru(NH(3))(6)(3+/2+) and Fe(CN)(6)(3-/4-). This established that a moderate collagen treatment does not incur any significant alteration of voltammetric responses or degradation of the excellent signal-to-noise ratio. Among these three microdevices, the most versatile one involved a configuration in which the ITO microelectrodes were delimited by a microchannel coiled into a spiral. Though providing extremely good electrochemical responses this specific design allowed proper seeding and culture of cells permitting either single cell or cell cluster stimulation and analysis.</AbstractText><CopyrightInformation>Copyright © 2011 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Meunier</LastName><ForeName>Anne</ForeName><Initials>A</Initials><Affiliation>Ecole Normale Supérieure, Département de Chimie, UMR CNRS-ENS-UPMC « PASTEUR », Paris, France.</Affiliation></Author><Author ValidYN="Y"><LastName>Fulcrand</LastName><ForeName>Rémy</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Darchen</LastName><ForeName>François</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Guille Collignon</LastName><ForeName>Manon</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Lemaître</LastName><ForeName>Frédéric</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Amatore</LastName><ForeName>Christian</ForeName><Initials>C</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType>Journal Article</PublicationType><PublicationType>Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2011</Year><Month>12</Month><Day>24</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Biophys Chem</MedlineTA><NlmUniqueID>0403171</NlmUniqueID><ISSNLinking>0301-4622</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Tin Compounds</NameOfSubstance></Chemical><Chemical><RegistryNumber>71243-84-0</RegistryNumber><NameOfSubstance>indium tin oxide</NameOfSubstance></Chemical><Chemical><RegistryNumber>9007-34-5</RegistryNumber><NameOfSubstance>Collagen</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="ErratumIn"><RefSource>Biophys Chem. 2013 Jan;171:84-5</RefSource></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Cell Line</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Collagen</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Electrochemical Techniques</DescriptorName><QualifierName MajorTopicYN="Y">instrumentation</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Equipment Design</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="Y">Exocytosis</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Microelectrodes</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Microtechnology</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Signal-To-Noise Ratio</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Single-Cell Analysis</DescriptorName><QualifierName MajorTopicYN="Y">instrumentation</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Surface Properties</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Tin Compounds</DescriptorName><QualifierName MajorTopicYN="Y">chemistry</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2011</Year><Month>10</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2011</Year><Month>12</Month><Day>9</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2011</Year><Month>12</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="aheadofprint"><Year>2011</Year><Month>12</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2012</Year><Month>1</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>1</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2012</Year><Month>6</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pii">S0301-4622(11)00299-7</ArticleId><ArticleId IdType="doi">10.1016/j.bpc.2011.12.002</ArticleId><ArticleId IdType="pubmed">22257976</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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